A proteomic approach is undertaken aiming at the identification of novel proteins involved in the alkaloid biosynthesis of Catharanthus roseus. Catharanthus roseus is a plant that has been utilised since ancient times to treat and manage various ailments such as DM . Production and metabolic engineering of terpenoid indole alkaloids in cell cultures of the medicinal plant Catharanthus roseus (L.) G. Don (Madagascar periwinkle). 11, 10 DECEMBER 2016 1811 *For correspondence. Jiayi Sun 1 0000139741 00000 n
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It is also commonly grown for its ornamental values and, due to ease of infection and distinctiveness of symptoms, is often used as the host for studies on phytoplasmas, an important group of uncultivated … Catharanthus roseus is the sole commercial source of the monoterpenoid indole alkaloids (MIAs), vindoline and catharanthine, components of the commercially important anticancer dimers, vinblastine and vincristine. Secondary metabolites are present in its leaves (e.g., vindoline, vinblastine, catharanthine, and vincristine) as well as basal stem and roots … 0000143730 00000 n
| Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. It is native and endemic to Madagascar, but grown elsewhere as an ornamental and medicinal plant, a source of the drugs vincristine and vinblastine, used to treat … Extraction yield (µg per g dry of plant material) of catharanthine and vindoline from Catharanthus roseus with the different solvents. Nowadays, synthesis of metal NPs is an interesting area in nanoscience. Physicochemical characterization was performed by Expasy’s Protparam server. (e-mail: dr.g.madhumitha@gmail.com) Catharanthus roseus-mediated zinc oxide nanoparticles against photocatalytic The methanolic whole plant extract at high dose (500 mg/kg) exhibited significant anti-hyperglycemic activity than whole plant extract at low dose (300 mg/kg) in diabetic rats. Catharanthus roseus L. (G. Don) leaf disks were transformed by cocultivation with seven strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. A double-flowered mutant was identified from the ‘Pacifica Polka Dot’ population. Results show that C. roseus is highly preferred by the majority (60%) of the interviewed healers, and that the pink form is exclusively used to treat gonorrhoea. Amongst these, two of the leaf-derived bisindole alkaloids—vinblastine and vincristine—are widely used in several anticancer chemotherapies. Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. 0
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The medicinal plant, Catharanthus roseus (C. roseus), accumulates a wide range of terpenoid indole alkaloids (TIAs). To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. 0000143590 00000 n
European herbalists used the plant for conditions as varied as headache to a folk remedy for diabetes. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. Seeds of Catharanthus roseus ‘Pacifica Polka Dot’ were purchased from PanAmerican Seed and 900 seeds were sown at Taoyuan District Agricultural Research and Extension Station (TDARES). 0000112931 00000 n
Epub 2016 Mar 17. Identification: orange - vindoline, blue – catharanthine. Comparison of the primary structure of the encoded protein with the amino-terminal sequence of purified SSS indicated the … 0000069530 00000 n
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(e-mail: dr.g.madhumitha@gmail.com) Catharanthus roseus-mediated zinc oxide nanoparticles against photocatalytic application of phenol red under UV@ 365 nm Aasaithambi Kalaiselvi1, Selvaraj Mohana Roopan2, Gunabalan Madhumitha2,*, Little Bright Eye) was achieved by HPLC-DAD-ESI-MS/MS. It shows a number of therapeutic activities like antimicrobial, antidiabetic, anticancer, antiallergic, anti-inflammatory, cardioprotective activity etc. The complete mRNA sequence for the enzyme strictosidine synthase (SSS) was determined. There are four important MIAs, vinblastine and vincristine used in the clinic as anti-cancer agents (Aslam et al., 2010), catharanthine which can reduce blood sugar content (Pan et al., 2012), and vindoline. Research article; Open Access; Published: 06 May 2016 Examining the transcriptional response of overexpressing anthranilate synthase in the hairy roots of an important medicinal plant Catharanthus roseus by RNA-seq. | 0000091658 00000 n
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Catharanthus roseus (C. roseus) is an important medicinal plant distributed in many countries. 0000003953 00000 n
The present study was carried out to evaluate the antidiabetic activity of Vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. Email: benjamin.philmus@oregonstate.edu (B. P.) and megrawm@science.oregonstate. C. roseus is a rich source of indole alkaloids. This study documented the antidiabetic efficacy of the combination of C. roseus and UA in rats. KEY POINTS: • C. roseus yields anticancerous vincristine and vinblastine used in pharma industry. HHS Lee ON, Ak G, Zengin G, Cziáky Z, Jekő J, Rengasamy KRR, Park HY, Kim DH, Sivanesan I. Molecules. COVID-19 is an emerging, rapidly evolving situation. 2009 Apr;52(Pt 4):313-23. doi: 10.1042/BA20080239. Screening of the phenolic compounds from seeds, stems, leaves and petals of Catharanthus roseus (L.) G. Don (cv. Muriel Le Roux, Françoise Guéritte, in Navelbine® and Taxotere®, 2017. Periwinkle [Catharanthus roseus (L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years ago.These alkaloidal drugs are still in clinical use. Catharanthus roseus L (apocyanaceae) also known as Vinca Rosea, is native to the Caribbean Basin and has historically been used to treat a wide assortment of diseases. 26, No. Remarkably, vinblastine and vincristine isolated from this plant … Carborundum abrasion technique was used to extract leaf epidermis–enriched mRNA, thus sampling the epidermome, or complement, of proteins expressed in the leaf epidermis. 0000082647 00000 n
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It also portrays the importance of various factors influencing the success of in vitro biotechnological interventions in Catharanthus and further addresses several shortcomings that can be further explored to create a platform for upcoming innovative approaches. 2020 Oct 26;25(21):4945. doi: 10.3390/molecules25214945. Catharanthus roseus belonging to family Apocynaceae is a plant with high medicinal importance and wide occurrence all over the world. The medicinal plant, Catharanthus roseus (C. roseus), accumulates a wide range of terpenoid indole alkaloids (TIAs). Carborundum abrasion technique was used to extract leaf epidermis–enriched mRNA, thus sampling the epidermome, or complement, of proteins expressed in the leaf epidermis. 2016 Oct;54(10):2033-43. doi: 10.3109/13880209.2016.1140213. The C. roseus cell suspension culture A11 accumulates the terpenoid indole alkaloids strictosidine, ajmalicine and vindolinine. The production of nanoparticles (NPs) for the manufacturing of new smart materials at the nanoscale with unique properties has rapidly increased in the past few years (Albrecht et al., 2006; Singh et al., 2016). 0000142961 00000 n
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A white C. roseus flower Catharanthus roseus, commonly known as bright eyes, Cape periwinkle, graveyard plant, Madagascar periwinkle, old maid, pink periwinkle, rose periwinkle, is a species of flowering plant in the family Apocynaceae. 0000071743 00000 n
• Key bottlenecks and prospective research methodologies have been identified herein. 0000142294 00000 n
Ethylene (ET) and methyl-jasmonate (MeJA) were previously reported as effective elicitors for the production of various valuable secondary metabolites of C. roseus, while a few ET or MeJA induced transcriptomic research is yet reported on this species. 0000078451 00000 n
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Pharmacognosy of Catharanthus roseus (L.) G. Don (Sadabahar) Root and its Adulterants. 0000071425 00000 n
The great pharmacological values, low in planta occurrence, unavailability of … At pH 5.0, 1.7 mg of alkaloid/g dry weight was produced. Periwinkle [Catharanthus roseus(L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years … 0000076916 00000 n
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Ԙ�Ŷhq��>�Xl� ��&�*�~��զ�5 C. roseus has been widely commercialized as an ornamental plant. Keywords: Endangered in the wild, it is a fast-growing plant that is easy to cultivate. 0000141471 00000 n
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Four distinct transformed cell lines were obtained with A. tumefaciens strains A281, BO542, and A. rhizogenes strain K599, and selected on hormone-free medium. 111, NO. Research Article Catharanthus roseus Combined with Ursolic Acid Attenuates Streptozotocin-Induced Diabetes through Insulin Secretion and Glycogen Storage Huda Mohammed Alkreathy 1 and Aftab Ahmad 2 1Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Catharanthus roseus (The Madagaskar Periwinkle) plant is commercially valued for harbouring more than 130 bioactive terpenoid indole alkaloids (TIAs). 0000069849 00000 n
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Clipboard, Search History, and several other advanced features are temporarily unavailable. Vinblastine and vincristine are two important anti-cancer drugs that are synthesized by the Terpenoid Indole Alkaloids (TIAs) pathway in periwinkle (Catharanthus roseus). Materials and Methods. Rats were divided into six groups. 0000082459 00000 n
Four distinct transformed cell lines were obtained with A. tumefaciens strains A281, BO542, and A. rhizogenes strain K599, and selected on hormone-free medium. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. It is popularly known as Madagascar periwinkle. 0000075567 00000 n
is a well known medicinal plant. 0000082703 00000 n
Catharanthus roseus (L.) G. Don a is known as the Madagascan periwinkle or the tropical periwinkle. 0000070639 00000 n
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